+ EN 13727

CHEMICAL DISINFECTANTS AND ANTISEPTICS. QUANTITATIVE SUSPENSION TEST FOR THE EVALUATION OF BACTERICIDAL ACTIVITY IN THE MEDICAL AREA

EN 13727 is a phase 2 step 1 suspension test to evaluate the bactericidal activity of disinfectants intended for use in the medical area under the conditions in which they are used. The test product is added to a test suspension of bacteria in a solution of an interfering substance. The mixture is maintained at a specified temperature for a specified contact time. The bactericidal action is immediately suppressed, the remaining viable bacteria are quantified and reductions are calculated.

+ EN 13697

CHEMICAL DISINFECTANTS AND ANTISEPTICS. QUANTITATIVE NON-POROUS SURFACE TEST FOR THE EVALUATION OF BACTERICIDAL AND/OR FUNGICIDAL ACTIVITY OF CHEMICAL DISINFECTANTS USED IN FOOD, INDUSTRIAL, DOMESTIC AND INSTITUTIONAL AREAS

EN 13697 is a phase 2 step 2 surface disinfection test to evaluate the bactericidal and/or fungicidal activity of disinfectants intended for use in food, industrial, domestic and institutional areas under the conditions in which they are used. A test suspension of bacteria or fungi in a solution of interfering substances is inoculated onto a test stainless steel disc and dried. The test product is applied in a manner which covers the dried film. The surface is maintained at a specified temperature for a specified contact time. The bactericidal/fungicidal action is immediately suppressed, the remaining viable bacteria/fungi are quantified and reductions are calculated.

+ ASTM E2197

STANDARD QUANTITATIVE DISK CARRIER TEST METHOD FOR DETERMINING BACTERICIDAL, VIRUCIDAL, FUNGICIDAL, MYCOBACTERICIDAL AND SPORICIDAL ACTIVITIES OF CHEMICALS

ASTM E2197 is a surface disinfection test to evaluate the bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activity of chemical disinfectants on nonporous surfaces. To test for bactericidal activity, a test suspension of bacteria including interfering substance is inoculated on to the centre of a stainless steel disc and is dried under vacuum. Once dry, individual discs are placed in the bottom of a sterile vessel for testing. The test product is applied directly to the centre of the disc and held for a specified contact time. After the contact time the bactericidal action is immediately suppressed and the remaining viable bacteria are quantified. Reductions are calculated from differences of log bacteria before and after treatment with the test product.

+ AATCC TM100

ASSESSMENT OF ANTIBACTERIAL FINISHES ON TEXTILE MATERIALS

The AATCC 100 test method is used to determine the bactericidal activity of fabrics and textiles over a 24 hour contact time. A test suspension of bacterial is inoculated onto control and test fabric swatches. Bacteria are immediately recovered from control and test samples to determine initial recovery concentrations. After the 24 hour contact period the remaining viable bacteria are quantified and reductions are calculated relative to initial concentration and to the control fabric.

+ Log reduction assays

Log reduction assays can be used to assess the antimicrobial effectiveness of wound dressings and antimicrobial impregnated medical devices. Test methods are designed to include a control test sample so that log reductions for the active test sample can be calculated in comparison to the control.

+ EN 1276

CHEMICAL DISINFECTANTS AND ANTISEPTICS. QUANTITATIVE SUSPENSION TEST FOR THE EVALUATION OF BACTERICIDAL ACTIVITY OF CHEMICAL DISINFECTANTS AND ANTISEPTICS USED IN FOOD, INDUSTRIAL, DOMESTIC AND INSTITUTIONAL AREAS

EN 1276 is a phase 2 step 1 suspension test to evaluate the bactericidal activity of disinfectants intended for use in the food, industrial, domestic and institutional areas under the conditions in which they are used. The test product is added to a test suspension of bacteria in a solution of an interfering substance. The mixture is maintained at a specified temperature for a specified contact time. The bactericidal action is immediately suppressed, the remaining viable bacteria are quantified and reductions are calculated.

+ EN 14561

CHEMICAL DISINFECTANTS AND ANTISEPTICS. QUANTITATIVE CARRIER TEST FOR THE EVALUATION OF BACTERICIDAL ACTIVITY FOR INSTRUMENTS USED IN THE MEDICAL AREA

EN 14561 is a phase 2 step 2 surface disinfection test to evaluate the bactericidal activity of disinfectants intended for use in the medical area under the conditions in which they are used. A test suspension of bacteria in a solution of interfering substances is spread onto a glass slide and dried. After drying the glass slide is immersed into a sample of test product and maintained at a specified temperature for a specified contact time. The bactericidal action is immediately suppressed by submerging the glass slide into neutraliser, the remaining viable bacteria are quantified and reductions are calculated.

+ ASTM D7907

STANDARD TEST METHOD FOR THE DETERMINATION OF BACTERICIDAL EFFICACY ON THE SURFACE OF MEDICAL EXAMINATION GLOVES

ASTM D7907 is a test method used to determine the bactericidal activity of medical gloves. A test suspension of bacteria, including an interfering substance if requested, is inoculated onto a sample of the test glove and held in place by a coverslip. The glove is maintained for a specified contact time, following which the test glove and coverslip are transferred to neutraliser to suppress bactericidal action. The remaining viable bacteria are quantified and reductions calculated.

+ Minimum inhibitory concentration (MIC)

The MIC is the lowest concentration of an antimicrobial agent that prevents or inhibits the growth of bacteria. These assays are useful for screening a range of antimicrobials against different bacteria.

+ ASTM 2799

STANDARD TEST METHOD FOR TESTING DISINFECTANT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA BIOFILM USING THE MBEC ASSAY

ASTM E2799 is a test method used to evaluate biofilm susceptibility to disinfectants. The assay device consists of a plastic lid with 96 pegs and a corresponding receiver plate with 96 individual wells. Biofilm is established on the pegs and then transferred into a new receiver plate containing the test disinfectant. Following the desired contact time, the disinfectant is immediately neutralised and remaining viable bacteria is quantified. Efficacy is reported as the log reduction of viable bacteria. The 96 well plate design allows for multiple disinfectants and/or multiple concentrations to be tested simultaneously making it an efficient screening tool. Although the test method defines the parameters for growing a Pseudomonas aeruginosa biofilm, it can be used to grow other species of biofilm.

+ ASTM E2562

STANDARD TEST METHOD FOR QUANTIFICATION OF PSEUDOMONAS AERUGINOSA BIOFILM GROWN WITH HIGH SHEAR AND CONTINUOUS FLOW USING CDC BIOFILM REACTOR

ASTM E2562 defines the parameters for growing Pseudomonas aeruginosa biofilm under high shear in a CDC Biofilm Reactor. The biofilm generated in the reactor is suitable for efficacy testing, allowing for treatment in situ or removal of test coupons for individual treatment.